Fluorescent in situ Hybridization (FISH)


Fluorescence in situ Hybridization (FISH) can help identify subtle structural rearrangements, variant chromosomes, and sub-microscopic changes not readily discernable by classic cytogenetics. FISH relies on three technologies: cytogenetics, DNA hybridization and fluorescence microscopy to provide another level of laboratory testing. FISH utilizes DNA probes comprised of specific nucleic acid sequences tagged with a fluorescent marker. FISH testing can be ordered by FISH probe, gene or in a disease-specific FISH panel.

FISH Directory

FISH Panels and Probes


FISH Test Name Chromosome Component Genes Disease Association
BCR/ABL+9q34 9, 22 ABL, BCR, ASS Chronic Myeloid Leukemia
Acute Lymphocytic Leukemia
MDS panel 5, 7, 8, 20 D5S721-D5S23/CSF1R,D7S486/Cen7,
Cen 8,D20S108		 Myelodysplastic Syndrome
CLL panel 11,12,13,14,17 ATM, CCND1(BCL1).
Cen12, D13S319, LAMP1,IgH, p53		 Chronic Lymphocytic Leukemia
AML-1/ETO 8, 21 ETO, AML 1 Acute Myeloid Leukemia (M2)
APL 15, 17 PML, RARA Acute Promyelocytic Leukemia (M3)
Mantle Cell Lymphoma 11, 14 CCND1(BCL1), IgH Mantle Cell Lymphoma
Burkitt Lymphoma 8, 14 MYC, Cen 8, IgH Burkitt Lymphoma
Follicular Lymphoma 14, 18 IgH, BCL 2 Follicular Lymphoma
MM-MGUS panel 1,3,5, 9, 13, 14, 17 CEB108/T7:1QTEL10,
D5S721-D5S23/CSF1R, Cen 3, Cen 9, D13S319, LAMP1, IgH, p53 		Multiple Myeloma
Reflex MM  4,11, 14, 16 FGFR3, CCND1, IgH, MAF Prognostic Indicators in Multiple Myeloma
Inv 16 16 CBFB Acute Myeloid Leukemia
MLL 11 MLL AML and ALL
MALT1 18 MALT 1 B-cell Non-Hodgkin Lymphoma, Margin Zone B-cell Lymphoma
BCL6 3 BCL6 B-cell Lymphoma
ALK 2 ALK Anaplasic Large Cell Lymphoma
PDGFRa 4 FIPL1L1/CHIC2/PDGFRa HES, CEL
MM t(4;14) 4,14 FGFR3, IgH Multiple Myeloma, MGUS
MM t(11;14) 11,14 CCND1 (BCL1), IgH Multiple Myeloma, MGUS
MM t(14;16) 14,16 IgH, MAF Multiple Myeloma, MGUS
IgH 14 IgH Lymphoma
AML panel 5, 7, 8, 11, 15, 16, 17, 21 D5S721-D5S23/CSF1R,
 D7S486/Cen 7, Cen 8,
 ETO, MLL, PML, CBFB, RARA, AML-1		 Acute Myeloid Leukemia
MALT t(11;18) 11,18 AP12, MALT1 MALT Lymphoma
MYC 8 MYC B-ALL, NHL
NHL 2,3,8,11,14,18 ALK, BCL6, MYC, Cen 8,  CCND1(BCL1), IgH, BCL2, MALT1 Non-Hodgkin's Lymphoma
Bladder Cancer 3, 7, 17, 9 Cen 3, Cen 7, Cen 17, 9p21 Bladder Cancer
Breast Cancer 17 HER2, Cen 17 Breast Cancer


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Direct Harvest

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Fixed Tissue

FISH Gene Amplification

HER 2 Gene Amplification